Conclusions : the in - vitro method that partial double - strand dna with t7 wi l . - toi promoter sequence as template and synthesizing by t7 rna polymerase can product high yield , excellent purity shrna . lt is a convenient - , effective ^ low - cost method and fit for small rna synthesis and rna interference researches in ordinary laboratory 结论：以带t7启动子部分双链dna为模板，用t7rna聚合酶体外合成出的shrna产量较高，纯度较好，是一种简便、高效、低成本的短链rna的制备方法，适合于普通实验室用来进行短链rna的合成和rna干扰实验。
Addition of iptg to growing culture of the lysogen induces t7 rna polymerase , which in turn transcribe the target dna in the plasmid . in the presence of glucose and appropriate conditions such as temperature and concerntration of iptg , a 52kd protein with tryptopanase activity was expressed 摸索发酵条件，如改变培养温度和iptg浓度等，发现在30培养条件下， 0 . 2mmiptg诱导时，发酵液中的吲哚含量最高，表明低浓度的诱导剂或低温诱导有利于表达出有活性的色氨酸酶。
Rna polymerase i synthesizes the three largest rrnas . rna polymerase ii mainly produces mrna encoding proteins and most of sn rnas , and rna polymerase iii makes 5s rrna and trna , as well as a few small nuclear rnas . the transcription sites of the polymerases have been studied since early 1980s 真核生物细胞核中有三种rna聚合酶，即rna聚合酶、和，它们分别转录产生不同的rna ，其中rna聚合酶( pol )转录合成45srrna前体；聚合酶( pol )转录合成mrna前体及大多数snrna ；聚合酶( pol )转录合成5srrna 、 trna和一些小分子rna 。
Hcv ns5b protein is encoded by ns5b gene , an rna - dependent rna polymerase that is responsible for the viral rna synthesis . due to no corresponding enzymes in the host cell and the importance of ns5b protein in the replication , ns5b protein has been regarded as an ideal target of anti - hcv infection Hcvns5b基因编码的ns5b蛋白，具有rna依赖的rna聚合酶活性( rdrp ) ，是基因组复制的关键酶。出于ns5b蛋白在hcv基因组复制中的重要作用和宿主细胞中缺乏具有类似功能的相应酶类， ns5b蛋白是抗hcv研究中的理想靶位。
The ns5 protein of dengue virus is the largest molecule encoded by the virus genome with a molecule weight of 104 000 . recent research work indicates that the ns5 protein acts as the rna - dependent rna polymerase ( rdrp ) in virus which has the ability to recognize and bind its template rna to synthesize a complementary strand 近年来的研究表明， ns5蛋白具有rna依赖的rna聚合酶( rdrp )功能，即可以识别具有相对特异性的模板rna并与之结合，合成与模板互补的rna链，在病毒基因组复制过程中起关键作用。
In this dissertation , jurkat cells , hela cells and mouse spleen t lymphocytes were chosen as experimental materials to answer the question . with the aid of various techniques such as elisa , immuno - co - precipitation , indirect immunofluoresent co - localization , double - labeling immunoelectron microscopy and so on , the relationships of baf complex with nf1 / ctf and rna polymerase ii were careful observed and analyzed 本文以jurkat细胞、 hela细胞和小鼠脾t淋巴细胞为研究材料，通过酶联免疫吸附实验( elisa ) 、免疫共沉淀、免疫荧光共定位和免疫电镜双标记等实验，观察和分析了baf复合物与转录因子nf1 ctf和rna聚合酶在基因转录活动中的相互联系。
About 25 salt - induced proteins were identified by maldi - tof / ms , and finally the function of 17 induced proteins were determined , including heat - shock proteins ( groes , clp ) . abc transporter , rna polymerase ( p - subunit ) and enzymes involved in signal transduction and metabolism . meanwhile , 84 proteins were idifferentially expressed after salt shock 5 min and 50 min in late exponential phase 应用maldi - tof ms对30个盐胁迫蛋白进行分析，已初步确定17个诱导蛋白的功能，其中包括与盐胁迫相关的热激蛋白( groes 、 clp ) 、与代谢途径和信号传导等有关的酶，以及abc转运蛋白、转录调节蛋白、 rna聚合酶的亚基等。
It is inferred that its active transcription occurs in the same region , not the nucleoplasm . the result will help us to further comprehend the mechanism of rna polymerase transcription , the way of its transcripts processing and transport , and the structural and functional relationship among the three rna polymeraes 这一结果不仅直观地向人们表明了rna聚合酶在真核细胞核中的转录位点，而且对于人们进一步认识和理解rna聚合酶的转录机制、其转录产物的加工运输途径、以及真核细胞当中不同的rna聚合酶间的组织和调控关系都将有着重要的理论意义。
From these results , it is inferred that the active transcription of pol iii occurs in the nucleoli and its periphery region , but not the nucleoplasm . the result will help us to further comprehend the mechanism of rna polymerase iii transcription , the way of its transcripts processing and transport , and the structural and functional relationship among the three rnapolymeraes 本实验为rna聚合酶在真核生物细胞核中的转录位点提供了较为直接的证据，这对人们进一步了解rna聚合酶的转录机制、加工和运输过程及三种rna聚合酶之间的结构与功能关系具有重要的意义。
Up until now , the active sites of rna polymerase and transcription have been investigated thoroughly and the opinions were in line with each other . however , with the development and the application of new technologies since 1990s , the controversy arose on the transcription sites of rna polymerase 目前有关rna聚合酶和rna聚合酶的转录位点已经研究得较为透彻并且也得到了大家的公认，但是自90年代以来随着研究技术方法的不断改进和发展，在rna聚合酶转录位点的确认方面却存在着不小的分歧。
